P18. The tumour leukocyte infiltrate is the key predictor for therapeutic response to catumaxomab therapy
نویسندگان
چکیده
Patients and methods Fresh tumour samples from 27 CRC patients were used to prepare tumour spheroids. Autologous PBMCs were isolated by ficoll density gradient. Tumour spheroids co-cultured with or without PBMCs were treated with catumaxomab for 96h. The cellular composition of the spheroids and the therapeutic impact on epithelial cells and leukocytes were measured by FACS analysis. The metabolic activity of the co-cultures was determined by the ATP assay.
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Clinical Medicine Insights: Therapeutics
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